VRU Research and Development Journal Science and Technology

VRU Research and Development Journal Science and Technology Vol. 15 No. 1 (2020)

2020-04-29T20:57:13+07:00

ANTIOXIDATION ACTIVITY AND TYROSINASE INHIBITION ACTIVITY FROM CRUDE EXTRACTS OF PSIDIUM GUAJAVA L.

2020-04-29T22:16:12+07:00

The aim of present study is to screen the phytochemical, total phenolic content, total tannin content, total flavonoid content antioxidant activity and tyrosinase inhibition activity of guava leaves (Psidium guajava L.). The guava leaves were extraction by ethanol. The crude extracts from guava leaves were analized total phenolic content, total tannin content, total flavonoid content, antioxidant activity using DPPH radical scavenging method and tyrosinase inhibition activity using dopachrome method. In this study, the phytochemical screening of guava leaves extracts were flavonoids, coumarns, saponins, tannins and phlobatannins. The extracts showed the highest total phenolic content and total flavonoid content which was of about 138.89±0.04 mg GAE/g, 157.56±0.02 mg TAE/g 45.29±0.05 mg QE/g, respectively. The antioxidant activity using DPPH radical scavenging method extracts of guava leaves showed the highest antioxidant activity with EC₅₀values of 12.11±0.08 mg/mL. Tyrosinase inhibition activity using dopachrome method extracts showed tyrosinase inhibition activity with EC₅₀values of 10.09±0.04 mg/mL.

THE EFFECT OF REHABILITATION ON THE ACTIVITIES OF DAILY LIVING OF THE ELDERLY HOME PHOTHARAM DISTRICT, RATCHABURI PROVINCE

2020-04-29T22:16:12+07:00

This research aimed to study the effect of rehabilitation program on the daily living activities of the homebound elderly in Photharam District, Ratchaburi Province. It was used the two group pretest posttest design. The sample consisted of 80 homebound elderly. The subjects were divided into an experimental group of 40 subjects who received rehabilitation program and a control group of 40 subjects who received only routine care. The research instrument were 1) rehabilitation program 2) questionnaire with reliability was 0.79. Data were collected data between December 2019–February 2020. Data were analyzed using descriptive statistics, frequency, percentage, average, standard deviation and compare the differences in the experimental groups and control groups before and after receive the program with the paired t-test statistics and analyzing the data, comparing the average difference between the group before and after receive the program with the Independent t-test statistics.

The result showed that after receive the program, experimental group had higher levels of knowledge and daily living activities than before receive the program with statistical significance. The experimental group had knowledge and daily living activities were significantly higher than the control group. Suggestions of this research, public health officer can apply this rehabilitation program to improve health and increase daily living activities of the homebound elderly.

THE DEVELOPMENT OF CLASSROOM ATTENDANCE TRACKING SYSTEM VIA QR CODE: A CASE STUDY OF PAKSE TEACHER TRAININ COLLEGE

2020-04-29T22:16:12+07:00

The objectives of this study were: 1) to develop of classroom attendance tracking system via QR code: a case study Pakse Teacher Training College; 2) to evaluate the software’s and 3) to evaluate user satisfaction. The system developed by using PHP language and MySQL database. There were four users (1) administrator, (2) staff, (3) professors, and (4) students.

The result of evaluating the software from 5 experts found that the system has efficiency at the highest level. The research sample consisted of 45 people from 3 user groups; professors, staff, and students. The results revealed that the user’s satisfaction with the system was at the right level.

THE EGG WHITE SUBSTITUTION IN CURRY PUFF WITH CHICKEN FOR ELDERS

2020-04-29T22:16:13+07:00

This research aims to develop food from egg whites for the elderly. By searching for the type of snacks as a refreshment that the elderly ate in daily life with a group discussion method of 15 elderlies and list the types of those refreshment for 400 elderlies to choose the type of most popular snack. The research found that the type of snack that the elderly choose. The top number of snacks are Thai snacks such as curry puff, tapioca pork, pudding with coconut topping etc, and the researcher has chosen the curry puff menu. By using egg whites instead of chicken stuffed for the elderly. By studying the amount of egg white that is appropriate to substitute chicken in curry puff by using egg whites to replace chicken in the filling of 25%, 50% and 75%. Then evaluate the sensory quality in terms of color, odor, taste, texture and overall liking uses 50 elderlies tasting in Trang Province. With tasting method of 9 preference points
(9 points hedonic scale), found that 50% of egg white substitute in stuff of curry puff was accepted in the smell, taste and overall liking were the most significant. At the same time, the physical properties such as color and texture of egg whites substitute in other formulas are not significant. Considering the chemical composition, it was found that when adding egg whites to substitute chicken in curry puff, the protein content was increased. And the energy value tends to decrease However, the elderlies receive higher protein albumin compared to the control formula, which is a quality protein that the body of the elderly needs

FACTORS ASSOCIATED WITH STRESS AMONG EMPLOYEES IN A FACTORY, SARABURI PROVINCE

2020-04-29T22:16:14+07:00

The study design was to cross-sectional survey study. The purposes of this study were to examine the stress and factors associated with stress among employees in a factory, Saraburi province. The subjects were 152 employees, selected by a multistage random sampling technique. The instruments used for data collection were questionnaires consisted of socio demographic factors, organizational environment factors, stress management and employee stress (SPST-20 Thai version). Data analysis was performed by using descriptive statistics, including the frequency percentage, mean, standard deviation and inferential statistic included Chi-square test. The results indicated that the respondents had high stress level 47.31%, severe stress level 39.78% and middle stress level 12.91%. Chi-square test results show that ages, educational level, working period, stress management and organizational environment factors were significantly associated with stress among metrological center employees. As gender, average monthly income and job position were not significantly associated with stress among metrological center employees. Therefore, organizations should organize activities to promote stress management such as providing mental health counseling for employees, family day activities and creating a conducive work environment to reduce employee stress.

FACTORS ASSOCIATED WITH SELF-CARE BEHAVIORS OF THE ELDER WITH HYPERTENSION IN BURIRAM PROVINCE BY USING THE PRECEDE FRAMEWORK THEORY

2020-04-29T22:16:14+07:00

This cross-sectional survey in research aimed to study the factors related to self-care behaviors among elder patients with hypertension at Tambon Ban Donkiang health promoting hospital, Tombon Bankhu, Amphoe Napho, Buriram province. The systematic sampling was 130 elder patients with hypertension.The data were collected by general questionnaire include the knowledge about hypertension, access to health services, social support and self-care behaviors. The statistics used for data analysis were percentage, mean, standard deviation, chi - square, and pearson's product moment correlation coefficient. The results showed that the majority of the samples were female. Most of them had a high level of knowledge about 46.15% then 41.53% moderate knowledge and low knowledge about 12.32%. The overall 4 aspects of self-care behaviors was a good level (= 3.05, S.D. = 0.45) when considering in 4 aspects as a eating behaviors, stress management behaviors and drug use behaviors which found that all aspects were a good level. Eating (= 3.17, S.D. = 0.48), stress management (= 3.10, S.D. = 0.45) and drug use (= 3.70, S.D. = 0.36) but found that exercise were a moderate level (= 2.21, S.D. = 0.58). Hence, the factors related to self-care behaviors divided to be predisposing factors include knowledge, occupation; enabling factors were income, health services access and reinforcing factors was social support were significantly related to self-care behaviors. (p<.05).

ANALYSIS OF CHROMOSOME ABERRATION IN INTERPHASE AND METAPHASE CELLS USING FLUORESCENCE IN SITU HYBRIDIZATION TECHNIQUE

2020-06-17T10:42:04+07:00

Fluorescent in situ hybridization technique (FISH) is developed to detect chromosomal abnormalities that are not observed by chromosome banding techniques such as microdeletion, microduplication, chromosome aneuploidy and also used to detect the fusion gene in leukemia. either in metaphase or interphase cells. The objective of this study is to apply FISH technique to detect chromosome aberrations in patients with genetic disease using both interphase and metaphases phases. DiGeorge syndrome (22q11 microdeletion), chronic leukemia (t(9; 22) (q34;q11.2)) and Patau's syndrome (trisomy 13) were used as case study to perform. Patient bone marrow or blood specimens were collected from Srinagarind Hospital, Faculty of Medicine, Khon Kaen University. The interphase and metaphase cells were prepared from bone marrow or blood by with and without cell culture at 37 °C for 72 hours, respectively. The interphase cells were used to detect BCR / ABL fusion gene due to t(9; 22) (q34;q11.2) using dual color dual fusion translocation probe and Patau's syndrome with trisomy 13 in using Vysis LSI 13 RB1 (13q14) probe respectively. The metaphase cells were used to detect microdeletion at 22q11 using amplification-deletion probe in DiGeorge syndrome patient. The achieve results were shown in this study. This demonstrated that the interphase cells without cell culture can be used to perform genetic testing. Especially, it is suitable for urgent diagnosis and monitor percentage of fusion gene in chronic leukemia patients. Whereas, the architecture of all chromosomes was demonstrated in the metaphase. In conclusion, FISH technique using interphase or metaphase cells as specimens can be used in a routine genetic testing of chromosomal abnormalities.

THE DEVELOPMENT OF KNOWLEDGE BANK SYSTEM FOR AGRICULTURAL

2020-06-17T10:23:31+07:00

This research’s objectives were 1) to design and develop agricultural knowledge bank system, and 2) to assess the efficiency of agricultural knowledge bank system. The result of the research shows that the agricultural knowledge system consists of how to grow crops, how to fertilize, harvest products, and video for cultivation knowledge. Which helps facilitate the users of the system easier to search for more information. The result of the evaluation of the users’ satisfaction toward the agricultural knowledge bank system, overall, was at good level, accounted for 4.43 percent. When it was considered on the levels of satisfaction, it was found that the highest satisfaction level was on after usage of the system there were more understanding which was at the highest level, 4.70 percent. It was followed by the satisfaction level on the system was able to use as a convenient media for publicizing which was at the highest level as well, accounting for 4.65 percent. On the other hand, the least satisfaction level was on the comprehensive information on demand which was at high level, 4.10 percent.

EFFICACY OF GEL MIXED THE CRUDE EXTRACT OF HIPTAGE CANDICANS HOOK.F. IN INHIBITING PATHOGENIC BACTERIA

2020-06-17T10:22:54+07:00

This research aims to study the quality and efficacy of gel products mixed with Hiptage candicans Hook.f crude extracts in inhibiting pathogenic bacteria. There are five strains including Staphylococcus epidermidis TISTR 518, Staphylococcus aureus TISTR 1466, Staphylococcus aureus TISTR 2329, Streptococcus mutans D 14283 and Proteus vulgaris DMST 557. The crude extracts from leaves and stems of Hiptage candicans Hook.f are taken for pre-formulation study to find the minimal inhibitory concentration (MIC). After that, it developed into gel products and tested the antibacterial effect by the disc diffusion method as well as testing the stability of the gel products in accelerated conditions. The results showed that crude extracts from leaves and stems were effective in inhibiting all five strains of pathogenic bacteria, with the minimal inhibitory concentration between 3.9 -125 milligram per milliliters. Further, the crude extract of leaves and stems showed highest antimicrobial activity against S. epidermidis TISTR 518 with MIC = 3.90 mg/ml. In addition, it was found that the crude extract from leaves showed more effective in antimicrobial activity than crude extracts from stems. When developed as a gel product, gels mixed crude extracts from leaves and gels mixed with crude extracts from stems. It found that the two gels were well performing in inhibition of S. epidermidis TISTR 518, S. aureus TISTR 1466 and S. aureus TISTR 2329. These results indicating that the Hiptage candicans Hook.f crude extracts had promising potential to develop as a product to control bacteria that cause skin disease. However, when testing the gel product in accelerated conditions, it was found that increased pH and reduced antibacterial effectiveness.

EFFECT OF VERMICOMPOST ON GROWTH AND YIELD OF LYCOPERSICON ESCULENTUM

2020-04-29T22:16:17+07:00

The objectives of this research were to 1) study the effects of vermicompost on the growth of Lycopersicon esculentum, and 2) study the effects of vermicompost on the yield of Lycopersicon esculentum. The experimental design was a completely randomized design with 6 different treatments of fertilizer applications, with 3 replications for each of the 5 plants, including chemical fertilizer applications with vermicompost of 50, 100, 150, 200 and 250 grams per plant. Their height, the number of fruits, fruit weight per inflorescence, color, sweetness and firmness were measured and recorded. An analysis of variance using the F-test to determine the variability between the group means was conducted.

The results revealed that:

1) Growing Lycopersicon esculentum with 50 grams of vermicompost per plant gave the maximum height at 103.40 centimeters with significant differences at the statistical level of 0.05.

2) The production of Lycopersicon esculentum with 150 grams of vermicompost per plant gave the maximum number of fruits at 3.78 fruits per inflorescence, with significant differences at the statistical level of 0.05. Meanwhile, there were no effects of the vermicompost ratios on the freshness of the fruit per inflorescence. In addition, using 200 grams of vermicompost gave the maximum fruit size at 23.14 millimeters in width and 33.78 millimeters in length, with significant differences respectively at the level of 0.05. The chemical fertilizer gave the most sweetness as well as the most firmness at 9.05 brix and 1.85 newtons, with significant differences at the statistical level of 0.05. There were no effects of fertilizer ratios on fruit color. The use of 150 grams of vermicompost gave the maximum brightness value at 39.69. The chemical fertilizer gave the highest red color value of 34.15. In addition, the use of 100 grams of vermicompost gave the highest yellow color value of 27.38. Therefore, the use of vermicompost in the production of Lycopersicon esculentum can be considered to be a substitute for chemical fertilizers to reduce the cost of cultivation.