DETERMINATION OF LEAD CONTAMINATION IN COCOA-FLAVORED PROTEIN POWDER SUPPLEMENTS AVAILABLE IN THAILAND

Authors

  • NIRAMON BAIPOKTHONG -
  • Thunnawat Wattanaseth -

Keywords:

Protein powder supplement, Lead, Whey protein, Plant-based protein

Abstract

     This study was survey research aimed to determine the concentration of lead (Pb) in cocoa- and chocolate-flavored protein powder products available in Thailand and classify the results according to product types. This laboratory-based descriptive study selected 20 samples using purposive sampling, including 10 whey protein products and 10 plant-based protein products. Lead concentrations were determined using Inductively Coupled Plasma–Optical Emission Spectrometry (ICP-OES) according to the AOAC standard method.

     The results showed that all 20 samples had lead concentrations below the detection limit of the analytical method (LOD = 0.12 mg/kg). Both whey protein and plant-based protein groups showed Not Detected results in all samples. Therefore, differences in lead concentrations between the two product groups could not be assessed because all detected values were below the analytical detection limit

References

EFSA Panel on Contaminants in the Food Chain (CONTAM). Scientific Opinion on Lead in Food. EFSA Journal. 2010;8(4):1570. doi:10.2903/j.efsa.2010.1570.

Bandara SB, Towle KM, Monnot AD. A human health risk assessment of heavy metal ingestion among consumers of protein powder supplements. Toxicology Reports. 2020;7:1255-1262. doi:10.1016/j.toxrep.2020.08.001.

Flora G, Gupta D, Tiwari A. Toxicity of lead: A review with recent updates. Interdisciplinary Toxicology. 2012;5(2):47-58. doi:10.2478/v10102-012-0009-2.

Patrick L. Lead toxicity, a review of the literature. Part 1: Exposure, evaluation, and treatment. Alternative Medicine Review. 2006;11(1):2-22.

World Health Organization. Lead poisoning and health [Internet]. Geneva: World Health Organization; 2023 [cited 2026 Jul 11]. Available from: https://www.who.int/news-room/fact-sheets/detail/lead-poisoning-and-health.

Tchounwou PB, Yedjou CG, Patlolla AK, Sutton DJ. Heavy metal toxicity and the environment. EXS. 2012;101:133-164. doi:10.1007/978-3-7643-8340-4_6.

Briffa J, Sinagra E, Blundell R. Heavy metal pollution in the environment and their toxicological effects on humans. Heliyon. 2020;6(9):e04691. doi:10.1016/j.heliyon.2020.e04691.

Abt E, Robin LP. Perspective on cadmium and lead in cocoa and chocolate. Journal of Agricultural and Food Chemistry. 2020;68(46):13067-13079. doi:10.1021/acs.jafc.0c02131.

Abt E, Fong Sam J, Gray P, Robin LP. Cadmium and lead in cocoa powder and chocolate products in the United States. Journal of Agricultural and Food Chemistry. 2018;66(37):9493-9501.

Association of Official Analytical Chemists (AOAC). Official Methods of Analysis of AOAC International. 21st ed. Rockville, MD: AOAC International; 2019.

United States Environmental Protection Agency (USEPA). Method 200.7: Determination of metals and trace elements in water and wastes by inductively coupled plasma-atomic emission spectrometry. Revision 4.4. Cincinnati: Environmental Monitoring Systems Laboratory; 1994.

Thompson M, Ellison SLR, Wood R. Harmonized guidelines for single-laboratory validation of methods of analysis. Pure Appl Chem. 2002;74(5):835-855.

Codex Alimentarius Commission. General Standard for Contaminants and Toxins in Food and Feed (CXS 193-1995). Rome: Food and Agriculture Organization of the United Nations/World Health Organization; 2023.

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Published

2026-09-30

How to Cite

BAIPOKTHONG, N., & Wattanaseth, T. (2026). DETERMINATION OF LEAD CONTAMINATION IN COCOA-FLAVORED PROTEIN POWDER SUPPLEMENTS AVAILABLE IN THAILAND. Journal of Environmental Education Medical and Health, 11(3), 168–173. retrieved from https://so06.tci-thaijo.org/index.php/hej/article/view/298229