The Effect of Cannabis Leave Tea and Grape Juice Ratio on Physical, Chemical and Microbiological Properties of Fruit Jelly

Abstract

The purpose of this research was to develop fruit jelly mixed with cannabis leaf tea that adheres to established safety and quality standards for consumer consumption. Four distinct formulations were examined, denoted as Formula 1, comprising 100% cannabis leaf tea; Formula 2, consisting of a blend of cannabis leaf tea and grape juice in a 70:30 ratio; Formula 3, combining cannabis leaf tea and grape juice in a 50:50 ratio; and Formula 4, integrating cannabis leaf tea and grape juice in a 30:70 ratio. Furthermore, the antioxidant capacity and tetrahydrocannabinol (THC) content were investigated.

The results revealed that all four formulas of fruit jelly mixed with cannabis leaf tea have the physical appearance of a soft jelly-like substance when taken out of the package. The texture is soft, flexible, and not hardened. The jellys had a clear natural color depending on the grape juice content and had a good hardening and stable texture when left at room temperature for 18–24 hours. In terms of chemical properties, the pH values ranged from 3.22 to 6.39, total acid content ranged from 0.2 to 0.45 percent, and % DPPH radical scavenging activity ranged from 34.85 to 48.63 percent. Notably, Formula 4 exhibited the highest total acid content and antioxidant activity among the formulations. The THC contents were evaluated by the cannabis test kit (Test Kann) of the Department of Medical Sciences, Ministry of Public Health. It was found that all four formulations of fruit jelly mixed with cannabis leaf tea products contained THC less than 0.2% (w/w).