EFFECT OF SOLVENT TYPE ON THE BIOACTIVITY OF CRUDE EXTRACT FROM TRADESCANTIA FLUMINENSIS
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Abstract
This study evaluated solvent effects on bioactive compound extraction from Tradescantia fluminensis using ultrasound-assisted extraction. Three solvents (ethanol, methanol, acetone) were compared for their extraction efficiency and biological activities. Ethanolic extract showed highest total phenolic (26.51 ± 0.44 mg GAE/g) and flavonoid (33.71 ± 0.50 mg QE/g) contents, with maximum antioxidant activity (IC50 = 10,530 ± 0.43 µg/mL). Both ethanol and methanol extracts demonstrated optimal antibacterial activity against Gram-positive bacteria Staphylococcus aureus and Bacillus subtilis (MIC = 7,810 µg/mL) and significant anti-inflammatory properties (IC50 = 22.20 ± 0.10 µg/mL) while maintaining cell viability above 90%. Results indicate that solvent polarity significantly influences bioactive compound recovery, with ethanol providing optimal extraction of therapeutic compounds from T. fluminensis. The results highlight the potential of this plant species as a valuable source of bioactive compounds for the development of phytopharmaceutical products and functional health applications.
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