A COMPARISON OF THE ANTIBACTERIAL ACTIVITY OF CHITOOLIGOSACCHARIDES PRODUCED BY CHITINASE AND CHITOSANASE EXTRACT FROM SEEDLINGS OF LEUCAENA LEUCOCEPHALA (LAM.) DE WIT
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Abstract
This research aimed to compare the antibacterial activity of chitooligosaccharides (COS) produced by chitinase and chitosanase extracted from two-week-old seedlings of Leucaena leucocephala de Wit. It was observed that COS obtained from chitinase through the hydrolysis of 1 percent weight per volume of colloidal chitin for 0.5 hours contained a percentage of larger molecules such as (GlcNAc)5, (GlcNAc)6, (GlcNAc)7, and (GlcNAc)8, which made up 93.74% of the total. This COS exhibited the strongest inhibition against six bacterial strains, including gram-positive bacteria such as B. cereus ATCC 11778, L. monocytogenes ATCC 15313, and S. aureus ATCC 25923, as well as gram-negative bacteria like E. coli ATCC 25922, P. aeruginosa ATCC 27853, and V. parahaemolyticus ATCC 17802. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were both measured at 0.195/0.195, 0.390/0.390, 0.195/0.195, 0.390/0.390, 0.390/0.390, and 0.390/0.390 μg/mL, respectively. For chitosanase, COS obtained from the hydrolysis of one percent weight per volume of chitosan for 0.5 hours contained a percentage of total larger molecules such as (GlcN)5, (GlcN)6, (GlcN)7, and (GlcN)8, accounting for 94.09% of the total. This COS exhibited the strongest inhibition against six bacterial strains, including gram-positive bacteria such as B. cereus ATCC 11778, L. monocytogenes ATCC 15313, and S. aureus ATCC 25923, as well as gram-negative bacteria like E. coli ATCC 25922, P. aeruginosa ATCC 27853, and V. parahaemolyticus ATCC 17802. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were both measured at 0.195/0.195, 0.390/0.390, 0.195/0.195, 0.390/0.390, 0.390/0.390, and 0.390/0.390 μg/mL, respectively. Therefore, it can be concluded that COS obtained by digesting one percent chitosan by mass per volume with chitosanase for 0.5 hours exhibited better antibacterial activity than COS obtained by digesting one percent colloidal chitin by mass per volume with chitinase enzyme for 0.5 hours.
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References
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