พิษกึ่งเฉียบพลัน พิษเฉียบพลันและองค์ประกอบทางเคมีของสารสกัดหยาบจากใบสดแป๊ะตำปึง

บทคัดย่อ

The work aimed to 1) study subacute toxicity against weight of Spraque-Dawley’s rats after received crude extract. 2) acute toxicity was studied against weight of mices after received crude extract and 3) determined chemical composition of Gynura divaricata DC. leaves crude extract. Fresh leaves of Gynura divaricata DC were macerated and extracted with ethanol.       The crude extract was studied subacute toxicity in streptozotocin induced diabetese Spraque-Dawley’s rats and acute toxicity in mices. Chemical composition of Gynura divaricata DC. fresh leaves crude extract was separated and analyzed by GC-MS.

The results showed as follow:

1. There is no subacute toxicity against weight of rats after feed fresh leaves crude extract. There is no difference in average weight of the rats that feed crude extract 2.9 µg/g b.w as compared with normal control and compared control groups. It was found that the weight of rats in diabetes control and the experimental groups that feed 5.79 and 11.43 µg/g b.w. significantly decreased (p<0.05) (249.6±14.5*, 270.2±61.9* and 289.0±43.9* respectively).

2. There is no acute toxicity found [n Gynura divaricata DC. fresh leaves crude extract.The LD₅₀ of Gynura divaricata DC. crude extract is more than 8 gm/kg which is 2,800 times of the 200 mg/70 kg. b.w.

3. The Gynura divaricata DC. fresh leaves crude extract also had total tannin content as 2.17 mg of tannic acid/ g extract and total flavonoid content as 49.84 mg rutin/ g extract. Chemical composition of the extract were analyzed by GC-MS were : (+)-Aromadendrene ; 4, 7, 10-Cycloundecatriene, 1, 1, 4, 8-tetramethyl-, cis, cis, cis- ; delta-Cadinene ; 2-Pentadecanone, 6, 10, 14-trimethyl ; Hexadecanoic acid, ethyl ester ; Neophytadiene ; Octadecanoic acid  and 1, 19-Eicosadiene.