Effect of processing methods on α-amylase and pasting properties of cassava flour

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Usa Yonkoksung
Sunee Chotineeranat
Kuakoon Piyachomkwan
Klanarong Sriroth

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              Cassava flour is usually produced at household level by various methods and used for direct consumptions in many countries. In this study, cassava flour was prepared by different methods (peeled vs unpeeled, grated vs sliced, pressed vs unpressed and reconstitution of starch and fibrous residues) and their paste viscosity properties were investigated by a Rapid Visco Analyzer (RVA). The results suggest inconsistent paste properties, in particular peak (PV1) and final viscosity (FV1), as influenced by the method of preparation.  Paste viscosity properties of these flour samples were further determined in the presence of silver nitrate (AgNO3), i.e. an enzyme inhibitor and less variation in paste properties was observed. The degree of change in peak and final viscosity of flour (DP and DF), which implied the amount of enzyme activity was the lowest in the reconstituted flour, followed by grated-peeled-pressed sample. When considering the paste viscosity of flour without the effect of enzyme, the effect of processing methods on paste viscosity of cassava flour was still observed, presumably caused by the different chemical compositions. Reconstituted flour, with the highest starch content (94.5% dwb) had the highest peak viscosity (356 RVU) while sliced flour samples with the lowest starch contents (81.9-83.5% dwb) had the lowest peak viscosity (257-266 RVU). 

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Author Biographies

Usa Yonkoksung

Ph.D. student, Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Chatuchak, Bangkok, Thailand. 

Sunee Chotineeranat

Cassava and Starch Technology Research Laboratory, National Center for Genetic Engineering and Biotechnology, Pathumthani, Thailand. 

Kuakoon Piyachomkwan

Cassava and Starch Technology Research Laboratory, National Center for Genetic Engineering and Biotechnology, Pathumthani, Thailand.

Klanarong Sriroth

Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Chatuchak, Bangkok, Thailand.

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