SCREENING AND IDENTIFICATION OF POLYUNSATURATED FATTY ACID PRODUCING BACTERIA BY SIMPLIFIED METHOD
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Abstract
Nowadays, polyunsaturated fatty acid (PUFAs) has been intensively interested since they possess the vital roles in the human health, implicated in pharmaceutical and health products. The objectives of this study were isolation of the PUFAs producing bacteria through the simplified method within short period of time based on an application of antioxidative effect of PUFAs against to the oxidizing agent. Here, the screening of PUFAs producing bacteria was performed by direct visualization using the 0.1% H2O2 plate assay. Five bacterial isolates including SS_Sa2, SS_Sa1.1, KT_W1Y, BK_PD3 and BK_SD4 were exhibited in the presence of H2O2 through the intracellular PUFA production. In addition, the bacterial identification was then confirmed by using 16S rDNA sequencing analysis followed by phylogenetic relationship evaluation. Five PUFAs producing bacteria were successfully classified as Pseudoxanthomanas sp., Bacillus subtilis, Burkholderia sp., Vibrio sp. and Pseudomonas fluorescens, respectively. Therefore, the results would be proposed the diverse bacterial strains toward PUFA production and considerably activation of PUFA production providing the further nutraceutical and pharmaceutical industrial.
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