QUANTITATION OF TOTAL PHENOLIC ANTIOXIDANT ACTIVITY AND DEVELOPMENT OF SKIN CARE LOTION

Abstract

This research study the total phenolic content and antioxidant activity from Crude extracts of Phyllanthus emblica, Elaeocarpus hygrophilus Kurz., Mimusops elengi L. and Morinda citrifolia L. were extracted with ethanol.  It was found that  the extract from Phyllanthus emblica. leaf has the highest value of 65.01 0.03 mg GAEg^-1. The extracts from Morinda citrifolia L., Elaeocarpus hygrophilus Kurz. and Mimusops elengi L. 43.85  0.01  31.25  0.02 and 22.08  0.01 mg GAEg^-1, respectively. When analyzed for antioxidant activity, crude extract from Phyllanthus emblica showed the highest antioxidant activity, with an IC₅₀ of 18.12 0.01 micrograms per milliliter. The extracts from Morinda citrifolia L., Elaeocarpus hygrophilus Kurz. and Mimusops elengi L. 14.84 0.01 12.91 0.02 and 0.92 0.01 micrograms per milliliter.  The extract of Phyllanthus emblica having the highest amount of total phenolic content and antioxidant activity were used as an ingredient in a lotion. Accelerated storage test on the lotion containing 0.5%w/w extract of Phyllanthus emblica was examined. The data were collected in every week for one month. At the temperature of 4^oC, 27^oC and the accelerate condition by light, the lotion was stable. At a temperature of 55^oC, the stability reduced in the 4^rd week. In the freeze and thaw cycle, the lotion showed the separation into layers in the 3^nd week. On the study of total phenolic and antioxidant activity, it was found that the lotion containing the extract from Phyllanthus emblica showed significantly higher amount of total phenolic compound than the basic lotion.