SCREENING, CHARACTERIZATION AND ANTIFUNGAL OF CHITOSANASE FROM THAI PLANTS

Abstract

To screening of chitosanase from Thai plants germinated 32 species in 21 Family Gramineae and 11 from Leguminosae. It found chitosanase from 32 plants germinated and were highly specific in the age of 2-4 weeks. Specific activity were 2.0812 - 32.9345 units/mg. There are 12 species that had specific activity more than 10 units/mg that specific activity from most to least are follows as: Sakon Nakhon rice, Khao Dawk Mali 105 rice, RD6 rice, Koshihikari rice, Pathum Thani 1 rice, Niaw San-pah-tawng 1 rice, Leuang Pratew 123 rice, Suphan Buri 1 rice, Suphan Buri 90 rice, Suphan Buri 60 rice, and RD8 rice, respectively. Chitosanase from two weeks of Sakolnakorn rice had the highest specific activity of 32.9345 U/mg and chitosanase from Acacia catechu Willd had the lowest activity was 2.0812 U/mg. Chitosanase from 32 plants germinated species had optimal pH and temperature of 3.5 to 6.0 and 40-60 C, respectively.
Chitosanase from 32 plants germinated species were inhibited fungi. Chitosanase from 12 plants germinated species with high specific activity were able to inhibit 7 species of fungi from 8 species by using chitosanase concentration to inhibit fungi of 10-15 μg.