ANTIOXIDANT ACTIVITY BY CHITOOLIGOSACCHARIDES OF CHITOSANASE FROM SAMANCA SAMAN (JACQ) MERR., LEUCAENA LEUCOCEPHALA DE WIT, ORYZA SATIVA RD. 6 AND SORGHUM VULGARE KU 630

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Abstract

This research was aimed to study the antioxidant activity by chitooligosaccharides of chitosanase from two weeks seedlings, Samanca saman (Jacq) Merr., Leucaena leucocephala de wit,  Oryza sativa RD. 6 and Sorghum vulgare KU 630 with 0.1 molar acetate buffer. The specific activity were 0.0061-10.8040 units/mg. The chitosanase from Samanca saman (Jacq) Merr. had the highest specific activity and decreased from Leucaena leucocephala de wit,  Oryza sativa RD. 6 and Sorghum vulgare KU 630, respectively. The optimum pH were extracted from Samanca saman (Jacq) Merr., Leucaena leucocephala de wit, Oryza sativa RD. 6 and Sorghum vulgare KU 630 of 4.5, 3.5, 4.5 and 4.5, respectively. The optimum temperature were extracted from Samanca saman (Jacq) Merr., Leucaena leucocephala de wit,  Oryza sativa RD. 6 and Sorghum vulgare KU 630 of 45, 45, 45 and 65 °C, respectively. Chitooligosaccharides obtained from the digestion of chitosanase  which extracted from Samanca saman (Jacq) Merr., Leucaena leucocephala de wit, Oryza sativa RD. 6 and Sorghum vulgare KU 630 were used for digestion 30 min that will be chitooligosaccharides of 1-6 ((GlcN)1-6). The small molecules of (GlcN)2 and (GlcN)3 were increased when the incubation time is increased to 1, 2 and 4 hours, while (GlcN)4, (GlcN)5 and (GlcN)6 were decreased. The large molecules ((GlcN)4, (GlcN)5 and (Glc)6) of Samanca saman (Jacq) Merr. were incubated for 30 minutes that had the highest and Leucaena leucocephala de wit,  Oryza sativa RD. 6 and Sorghum vulgare KU 630, respectively. Chitooligosaccharides obtained from chitosanase


of Samanca saman (Jacq) Merr., Leucaena leucocephala de wit,  Oryza sativa RD. 6 and Sorghum vulgare KU 630 can antioxidant when tested using DPPH and 1,10 phenanthrolene. In order of which Leucaena leucocephala de wit, Samanca saman (Jacq) Merr., Oryza sativa RD. 6 and Sorghum vulgare KU 630. Chitooligosaccharides from Samanca saman (Jacq) Merr. and Leucaena leucocephala de wit were able to antioxidant better than BHT when compared with the EC50.

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