ANTIOXIDANT ACTIVITIES AND TOTAL PHENOLIC CONTENTS OF CRUDE ETHANOLIC EXTRACT FROM MATAT (DILLENIA INDICA)

Abstract

Dillenia indica (Matat)) is locally plant in Pathumthani. The fruits are normally used as flavoring agent for curries. Matat plants are used as decoration and locally medicine. The objectives of this research were to analyze the amount of total phenolic and to evaluate antioxidant activities of all crude extracts using DPPH and FRAP assay. Both peel and pulp of fresh and dried Matat were studied. Matat fruits were collected from the village in Pathumthani Province. Fresh Matat fruits were dried at 40-45 ^OC for 48 hours. It was found that the level of moisture from both peels and flesh of fresh Matat were 88.40% and 69.19%, respectively. The percentage yield of crude extracts from dried Matat flesh was the highest (19.06%), whereas the lowest percentage yield of crude extracts was from fresh Matat peels (2.34%). The total phenolic contents of the crude extracts were determined by the Folin–Ciocalteu method using gallic acid as the standard solution. It was found that the level of total phenolic content of crude extracts of both peels and flesh of fresh and dried Matat were 70.91, 10.29, 67.10, and 80.34 mg gallic acid equivalent/ g sample, respectively. The test of antioxidant activities from both peels and flesh of fresh and dried Matat in ethanol via DPPH method was performed. The IC50 values of the fresh and dried Matat peels were obtained at 0.017 mg/ml and 0.26 mg/ml, respectively. By contrast, the IC50 values of the fresh and dried Matat flesh were 0.019 mg/ml and 0.004 mg/ml, respectively. Finally, the IC50 values of the test of antioxidants via FRAP method were 79.634, 0.135, 73.388, and 144.785 mgEqFeSO4 / g sample, respectively. These finding suggest that the extract of dried fruit pulp and friut peels of Matat have high content of phenols and flavonoid may presented the potent antioxidant and anticancer activities. Further study should be determinated.