TOTAL PHENOLIC CONTENT, TOTAL FLAVONOIDS CONTENT, ANTIOXIDANT ACTIVITY AND ALPHA GLUCOSIDASE INHIBITORY ACTIVITIES OF ETHANOLIC EXTRACT OF BORASSUS FLABELLIFER L. MALE FLOWERS
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Abstract
This research aimed to screen the phytochemical, total phenolic content, total flavonoid content, antioxidant activity and anti α-glucosidase activity of ethanolic extract of Borassus flabellifer L. male flowers. The Inhibition of the enzyme α-glucosidase was used to maintain post-meal blood glucose levels in diabetes mellitus type 2 patients. The results showed that the phytochemical screening of ethanolic extract of Borassus flabellifer L. male flowers were flavonoids, coumarins, saponins and terpenoids. The extracts showed the total phenolic contents and total flavonoid contents of 386.07±11.16 mgGAE/gextract and 637.63±21.28 mgQE/gextract, respectively. The antioxidant activity of ethanolic extract of Borassus flabellifer L. showed the IC50 values of 0.039±0.005 mg/mL. The extract was not statistically significant difference from butylated hydroxytoluene at the 95% confidence level. The results of anti-glucosidase activity, maltase and sucrase, showed the IC50 values of 218.48±0.55 and 286.49±0.09 µg/mL, respectively. In conclusion, the ethanolic extract of Borassus flabellifer L. male flowers can be used as a natural source of antioxidants and it can be developed as an alternative supplementary to control blood sugar levels.
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