ANTIOXIDANT, ANTI-OPPORTUNISTIC BACTERIA AND SYNERGISTIC ACTION WITH OXYTETRACYCLINE AND AMPICILLIN OF Terminalia chebula CRUDE EXTRACT
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Abstract
Terminalia chebula Retz, a perennial plant, is commonly found in tropical area. T. chebula is a medicinal plant of which has many therapeutic effects. This research was aimed to study antioxidant activity, antimicrobial activity, and synergistic effect of fruit extract from Terminalia chebula by broth dilution susceptibility test Also, antioxidant activity was determined by DPPH radical scavenging activity (DPPH assay) and Ferric ion Reducing Antioxidant Power assay (FRAP assay). T. chebula fruit extract showed antioxidant activity with the IC50 of 0.018 mg/mL by DPPH assay. By FRAP assay, T. chebula fruit extract showed reducing power of Fe3+ to Fe2+ by 96.69±0.003 mgFeSO4/g extract. The results were shown that T. chebula fruit extract had antibacterial activity against Escherichia coli ATCC 25922, Klebsiella pneumoniae, Proteus mirabilis, Proteus vulgaris and Serratia marcescens with MICs ranging by 1.25-80 mg/ml. Synergistic action of T. chebula fruit extract in combination with oxytetracycline and ampicillin was investigated, and the results were shown that the synergism effect of T. chebula fruit extract combined with ampicillin showed additive effect against E. coli ATCC 25922, P. mirabilia, P. vulgaris, K. Pneumoniae. Finally, T. chebula fruit extract may be used as synergistic action when combined with oxytetracycline and ampicillin against opportunistic bacteria.
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