ISOLATION AND SCREENING OF MANNANASE PRODUCING BACTERIA FROM MANNAN WASTE
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Mannanase is the most important enzyme for the hydrolysis of mannan, which is produced by microorganisms, including bacteria and fungi. Mannanase could be used as valuable food, its application for mannooligosaccharides preparation as prebiotic. This study focus on the isolation and screening an effective strain of mannanase producing bacteria. The result found that 2 isolates showing mannanase production were isolated from soil samples, which compose of waste from mannan. Isolate P2-5 and P3-1 showed high ratio of clear zone diameter to colony diameter 1.75 and 1.50, respectively. Isolate P2-5 and P3-1 were identified by 16S rDNA gene sequencing, biochemical test and morphology. The results of bacterial identification revealed P2-5 and P3-1 as Bacillus subtilis, which were 99% identity. The both isolates were able to ferment some of carbohydrates such as D-glucose D-mannitol
D-mannose Myo-inositol D-ribose and D-trehalose. Bacillus species are an important source of mannanase enzymes production. Therefore, the both isolates will be used for mannanase enzyme production and its application for various industrials and mannooligosaccharides preparation.
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ลิขสิทธิ์บทความวิจัยที่ได้รับการตีพิมพ์เผยแพร่ในวารสารวิจัยและพัฒนา วไลยอลงกรณ์ ในพระบรมราชูปถัมภ์ ถือเป็นกรรมสิทธิ์ของสถาบันวิจัยและพัฒนา มหาวิทยาลัยราชภัฏวไลยอลงกรณ์ ในพระบรมราชูปถัมภ์ ห้ามนำข้อความทั้งหมดหรือบางส่วนไปพิมพ์ซ้ำ เว้นแต่จะได้รับอนุญาตจากมหาวิทยาลัยเป็นลายลักษณ์อักษร
ความรับผิดชอบ เนื้อหาต้นฉบับที่ปรากฏในวารสารวิจัยและพัฒนา วไลยอลงกรณ์ ในพระบรมราชูปถัมภ์ เป็นความรับผิดชอบของผู้นิพนธ์บทความหรือผู้เขียนเอง ทั้งนี้ไม่รวมความผิดพลาดอันเกิดจากเทคนิคการพิมพ์
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