ANTIOXIDANT ACTIVITY OF CRUDE EXTRACT FROM TERMINALIA CHEBULIA

Abstract

This research was to study antioxidant activity and separated the chemical compound of crude extract from crude extract from Terminalia chebula.  Samples were extracted with ethanol by immersion method while the quantity of total phenolic and total tannin.                            We conducted the investigation of the antioxidant activity of the crude extract via DPPH Radical Scavenging with UV-VIS Spectrophotometer. The results illustrated that the crude extract from samples had total phenolic contents at 85.22 milligram gallic acid/100 g FW, total tannin contents at 365.77 microgram/milliliter respectively whilst IC₅₀ value of the antioxidant activity was 13.24 milligram/milliliter and IC₅₀ value of BHA and BHT were 12.06 and 11.92 milligram/milliliter respectively. The separation of crude extract can be separated into four groups (F1, F2, F3, F4 and F5) and the results showed that F2 has the strongest activity against the others with IC₅₀ = 12.53 milligram/milliliter. The lotion was odorless, white color with no phase separation with pH 6.7